Yale won the doubles point but dropped a 4-1 decision to Penn at the Cullman-Heyman Tennis Center.
The Quakers, though, rallied in singles, winning at No. 1, No. 2, No. 4 and No. 6.
The Bulldogs wrap up the season by hosting Princeton on Sunday.
Kyle Mautner (P) def. Dylan King (Y) 6-4, 7-6
Edoardo Graziani (P) def. Cody Lin (Y) 6-4, 7-6(3)
Kevin Zhu (P) vs. Ryan Cheng (Y) DNF
Dmitry Shatalin (P) def. Arnav Dhingra (Y) 4-6, 7-6(4), 6-0
Noah Makarome (P) vs. Andrew Heller (Y) DNF
Max Cancilla (P) def. Alan Sou (Y) 7-5, 6-4
Lin-Cheng (Y) def. Mautner-Graziani (P) 6-4
Brown-king (Y) def. Zhu-Shatalin (P) 6-3
Heller-Dhingra (Y) vs. Cancilla-Makarome (P) DNF